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Image Search Results
Journal: The Kaohsiung Journal of Medical Sciences
Article Title: miR ‐450a exerts oncosuppressive effects in breast carcinoma by targeting CREB1
doi: 10.1002/kjm2.12547
Figure Lengend Snippet: miR‐450a is lowly expressed in breast cancer cells. (A) The expression levels of miR‐450a in breast cancer cells (MCF‐7, MDA‐MB‐231, MDA‐MB‐468, T47D, BT474, and SK‐BR‐3) and human breast epithelial cell line MCF‐10A were detected by RT‐qPCR. (B) T47D and BT474 cells were transfected with miR‐450a or NC, respectively. The transfection efficiency was determined by RT‐qPCR. (C,D) Upon miR‐450a mimic transfection, the cell proliferation of T47D and BT474 were measured using a CCK‐8 assay. (E,F) The growth of T47D and BT474 cells was assessed using colony formation assays. (G) Western blotting validation of the effects of miR‐450a on the phosphorylation of PI3K/AKT. ** P < 0.01, compared with NC
Article Snippet: The
Techniques: Expressing, Quantitative RT-PCR, Transfection, CCK-8 Assay, Western Blot, Biomarker Discovery, Phospho-proteomics